Intracellular amorphous carbonates uncover a new biomineralization process in eukaryotes.
Identifieur interne : 000934 ( PubMed/Checkpoint ); précédent : 000933; suivant : 000935Intracellular amorphous carbonates uncover a new biomineralization process in eukaryotes.
Auteurs : A. Martignier [Suisse] ; M. Pacton [France] ; M. Filella [Suisse] ; J-M Jaquet [Suisse] ; F. Barja [Suisse] ; K. Pollok [Allemagne] ; F. Langenhorst [Allemagne] ; S. Lavigne [Suisse] ; P. Guagliardo [Australie] ; M R Kilburn [Australie] ; C. Thomas [France] ; R. Martini [Suisse] ; D. Ariztegui [Suisse]Source :
- Geobiology [ 1472-4669 ] ; 2017.
Descripteurs français
- KwdFr :
- MESH :
- analyse : Carbonates.
- microbiologie : Lacs, Phytoplancton.
- métabolisme : Chlorophyta.
- Chlorophyta, Cytoplasme, Suisse, Voies et réseaux métaboliques.
English descriptors
- KwdEn :
- MESH :
- chemical , analysis : Carbonates.
- chemistry : Chlorophyta, Cytoplasm.
- metabolism : Chlorophyta.
- microbiology : Lakes, Phytoplankton.
- Metabolic Networks and Pathways, Switzerland.
Abstract
Until now, descriptions of intracellular biomineralization of amorphous inclusions involving alkaline-earth metal (AEM) carbonates other than calcium have been confined exclusively to cyanobacteria (Couradeau et al., 2012). Here, we report the first evidence of the presence of intracellular amorphous granules of AEM carbonates (calcium, strontium, and barium) in unicellular eukaryotes. These inclusions, which we have named micropearls, show concentric and oscillatory zoning on a nanometric scale. They are widespread in certain eukaryote phytoplankters of Lake Geneva (Switzerland) and represent a previously unknown type of non-skeletal biomineralization, revealing an unexpected pathway in the geochemical cycle of AEMs. We have identified Tetraselmis cf. cordiformis (Chlorophyta, Prasinophyceae) as being responsible for the formation of one micropearl type containing strontium ([Ca,Sr]CO3 ), which we also found in a cultured strain of Tetraselmis cordiformis. A different flagellated eukaryotic cell forms barium-rich micropearls [(Ca,Ba)CO3 ]. The strontium and barium concentrations of both micropearl types are extremely high compared with the undersaturated water of Lake Geneva (the Ba/Ca ratio of the micropearls is up to 800,000 times higher than in the water). This can only be explained by a high biological pre-concentration of these elements. The particular characteristics of the micropearls, along with the presence of organic sulfur-containing compounds-associated with and surrounding the micropearls-strongly suggest the existence of a yet-unreported intracellular biomineralization pathway in eukaryotic micro-organisms.
DOI: 10.1111/gbi.12213
PubMed: 27696636
Affiliations:
- Allemagne, Australie, France, Suisse
- Auvergne-Rhône-Alpes, Canton de Genève, Rhône-Alpes
- Genève, Lyon, Thonon-les-Bains, Villeurbanne
- Université de Genève
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pubmed:27696636Le document en format XML
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Martignier</name><affiliation wicri:level="4"><nlm:affiliation>Department of Earth Sciences, University of Geneva, Geneva, Switzerland.</nlm:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>Department of Earth Sciences, University of Geneva, Geneva</wicri:regionArea><orgName type="university">Université de Genève</orgName><placeName><settlement type="city">Genève</settlement><region nuts="3" type="region">Canton de Genève</region></placeName></affiliation></author><author><name sortKey="Pacton, M" sort="Pacton, M" uniqKey="Pacton M" first="M" last="Pacton">M. 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Barja</name><affiliation wicri:level="4"><nlm:affiliation>Microbiology unit, University of Geneva, Geneva, Switzerland.</nlm:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>Microbiology unit, University of Geneva, Geneva</wicri:regionArea><orgName type="university">Université de Genève</orgName><placeName><settlement type="city">Genève</settlement><region nuts="3" type="region">Canton de Genève</region></placeName></affiliation></author><author><name sortKey="Pollok, K" sort="Pollok, K" uniqKey="Pollok K" first="K" last="Pollok">K. 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Langenhorst</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Geosciences, Friedrich Schiller University Jena, Jena, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute of Geosciences, Friedrich Schiller University Jena, Jena</wicri:regionArea><wicri:noRegion>Jena</wicri:noRegion><wicri:noRegion>Jena</wicri:noRegion><wicri:noRegion>Jena</wicri:noRegion></affiliation></author><author><name sortKey="Lavigne, S" sort="Lavigne, S" uniqKey="Lavigne S" first="S" last="Lavigne">S. Lavigne</name><affiliation wicri:level="1"><nlm:affiliation>Service de l'Ecologie de l'Eau (SECOE), Geneva, Switzerland.</nlm:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>Service de l'Ecologie de l'Eau (SECOE), Geneva</wicri:regionArea><wicri:noRegion>Geneva</wicri:noRegion></affiliation></author><author><name sortKey="Guagliardo, P" sort="Guagliardo, P" uniqKey="Guagliardo P" first="P" last="Guagliardo">P. Guagliardo</name><affiliation wicri:level="1"><nlm:affiliation>Centre for Microscopy, Characterisation and Analysis, The University of Western Australia, Perth, WA, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Centre for Microscopy, Characterisation and Analysis, The University of Western Australia, Perth, WA</wicri:regionArea><wicri:noRegion>WA</wicri:noRegion></affiliation></author><author><name sortKey="Kilburn, M R" sort="Kilburn, M R" uniqKey="Kilburn M" first="M R" last="Kilburn">M R Kilburn</name><affiliation wicri:level="1"><nlm:affiliation>Centre for Microscopy, Characterisation and Analysis, The University of Western Australia, Perth, WA, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Centre for Microscopy, Characterisation and Analysis, The University of Western Australia, Perth, WA</wicri:regionArea><wicri:noRegion>WA</wicri:noRegion></affiliation></author><author><name sortKey="Thomas, C" sort="Thomas, C" uniqKey="Thomas C" first="C" last="Thomas">C. Thomas</name><affiliation wicri:level="3"><nlm:affiliation>CARRTEL of Thonon-les-Bains, INRA, Thonon-les-Bains, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>CARRTEL of Thonon-les-Bains, INRA, Thonon-les-Bains</wicri:regionArea><placeName><region type="region">Auvergne-Rhône-Alpes</region><region type="old region">Rhône-Alpes</region><settlement type="city">Thonon-les-Bains</settlement></placeName></affiliation></author><author><name sortKey="Martini, R" sort="Martini, R" uniqKey="Martini R" first="R" last="Martini">R. Martini</name><affiliation wicri:level="4"><nlm:affiliation>Department of Earth Sciences, University of Geneva, Geneva, Switzerland.</nlm:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>Department of Earth Sciences, University of Geneva, Geneva</wicri:regionArea><orgName type="university">Université de Genève</orgName><placeName><settlement type="city">Genève</settlement><region nuts="3" type="region">Canton de Genève</region></placeName></affiliation></author><author><name sortKey="Ariztegui, D" sort="Ariztegui, D" uniqKey="Ariztegui D" first="D" last="Ariztegui">D. Ariztegui</name><affiliation wicri:level="4"><nlm:affiliation>Department of Earth Sciences, University of Geneva, Geneva, Switzerland.</nlm:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>Department of Earth Sciences, University of Geneva, Geneva</wicri:regionArea><orgName type="university">Université de Genève</orgName><placeName><settlement type="city">Genève</settlement><region nuts="3" type="region">Canton de Genève</region></placeName></affiliation></author></analytic><series><title level="j">Geobiology</title><idno type="eISSN">1472-4669</idno><imprint><date when="2017" type="published">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Carbonates (analysis)</term><term>Chlorophyta (chemistry)</term><term>Chlorophyta (metabolism)</term><term>Cytoplasm (chemistry)</term><term>Lakes (microbiology)</term><term>Metabolic Networks and Pathways</term><term>Phytoplankton (microbiology)</term><term>Switzerland</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Carbonates (analyse)</term><term>Chlorophyta ()</term><term>Chlorophyta (métabolisme)</term><term>Cytoplasme ()</term><term>Lacs (microbiologie)</term><term>Phytoplancton (microbiologie)</term><term>Suisse</term><term>Voies et réseaux métaboliques</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Carbonates</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Carbonates</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Chlorophyta</term><term>Cytoplasm</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Chlorophyta</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Lacs</term><term>Phytoplancton</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Lakes</term><term>Phytoplankton</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Chlorophyta</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Metabolic Networks and Pathways</term><term>Switzerland</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Chlorophyta</term><term>Cytoplasme</term><term>Suisse</term><term>Voies et réseaux métaboliques</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Until now, descriptions of intracellular biomineralization of amorphous inclusions involving alkaline-earth metal (AEM) carbonates other than calcium have been confined exclusively to cyanobacteria (Couradeau et al., 2012). Here, we report the first evidence of the presence of intracellular amorphous granules of AEM carbonates (calcium, strontium, and barium) in unicellular eukaryotes. These inclusions, which we have named micropearls, show concentric and oscillatory zoning on a nanometric scale. They are widespread in certain eukaryote phytoplankters of Lake Geneva (Switzerland) and represent a previously unknown type of non-skeletal biomineralization, revealing an unexpected pathway in the geochemical cycle of AEMs. We have identified Tetraselmis cf. cordiformis (Chlorophyta, Prasinophyceae) as being responsible for the formation of one micropearl type containing strontium ([Ca,Sr]CO3 ), which we also found in a cultured strain of Tetraselmis cordiformis. A different flagellated eukaryotic cell forms barium-rich micropearls [(Ca,Ba)CO3 ]. The strontium and barium concentrations of both micropearl types are extremely high compared with the undersaturated water of Lake Geneva (the Ba/Ca ratio of the micropearls is up to 800,000 times higher than in the water). This can only be explained by a high biological pre-concentration of these elements. The particular characteristics of the micropearls, along with the presence of organic sulfur-containing compounds-associated with and surrounding the micropearls-strongly suggest the existence of a yet-unreported intracellular biomineralization pathway in eukaryotic micro-organisms.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">27696636</PMID><DateCreated><Year>2016</Year><Month>10</Month><Day>03</Day></DateCreated><DateCompleted><Year>2017</Year><Month>09</Month><Day>05</Day></DateCompleted><DateRevised><Year>2017</Year><Month>09</Month><Day>06</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1472-4669</ISSN><JournalIssue CitedMedium="Internet"><Volume>15</Volume><Issue>2</Issue><PubDate><Year>2017</Year><Month>Mar</Month></PubDate></JournalIssue><Title>Geobiology</Title><ISOAbbreviation>Geobiology</ISOAbbreviation></Journal><ArticleTitle>Intracellular amorphous carbonates uncover a new biomineralization process in eukaryotes.</ArticleTitle><Pagination><MedlinePgn>240-253</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1111/gbi.12213</ELocationID><Abstract><AbstractText>Until now, descriptions of intracellular biomineralization of amorphous inclusions involving alkaline-earth metal (AEM) carbonates other than calcium have been confined exclusively to cyanobacteria (Couradeau et al., 2012). Here, we report the first evidence of the presence of intracellular amorphous granules of AEM carbonates (calcium, strontium, and barium) in unicellular eukaryotes. These inclusions, which we have named micropearls, show concentric and oscillatory zoning on a nanometric scale. They are widespread in certain eukaryote phytoplankters of Lake Geneva (Switzerland) and represent a previously unknown type of non-skeletal biomineralization, revealing an unexpected pathway in the geochemical cycle of AEMs. We have identified Tetraselmis cf. cordiformis (Chlorophyta, Prasinophyceae) as being responsible for the formation of one micropearl type containing strontium ([Ca,Sr]CO3 ), which we also found in a cultured strain of Tetraselmis cordiformis. A different flagellated eukaryotic cell forms barium-rich micropearls [(Ca,Ba)CO3 ]. The strontium and barium concentrations of both micropearl types are extremely high compared with the undersaturated water of Lake Geneva (the Ba/Ca ratio of the micropearls is up to 800,000 times higher than in the water). This can only be explained by a high biological pre-concentration of these elements. The particular characteristics of the micropearls, along with the presence of organic sulfur-containing compounds-associated with and surrounding the micropearls-strongly suggest the existence of a yet-unreported intracellular biomineralization pathway in eukaryotic micro-organisms.</AbstractText><CopyrightInformation>© 2016 John Wiley & Sons Ltd.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Martignier</LastName><ForeName>A</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Department of Earth Sciences, University of Geneva, Geneva, Switzerland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Pacton</LastName><ForeName>M</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Laboratoire de Géologie de Lyon, Lyon 1 University, Villeurbanne, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Filella</LastName><ForeName>M</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Institute F.-A. Forel, University of Geneva, Geneva, Switzerland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Jaquet</LastName><ForeName>J-M</ForeName><Initials>JM</Initials><AffiliationInfo><Affiliation>Department of Earth Sciences, University of Geneva, Geneva, Switzerland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Barja</LastName><ForeName>F</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>Microbiology unit, University of Geneva, Geneva, Switzerland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Pollok</LastName><ForeName>K</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>Institute of Geosciences, Friedrich Schiller University Jena, Jena, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Langenhorst</LastName><ForeName>F</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>Institute of Geosciences, Friedrich Schiller University Jena, Jena, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lavigne</LastName><ForeName>S</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Service de l'Ecologie de l'Eau (SECOE), Geneva, Switzerland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Guagliardo</LastName><ForeName>P</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>Centre for Microscopy, Characterisation and Analysis, The University of Western Australia, Perth, WA, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kilburn</LastName><ForeName>M R</ForeName><Initials>MR</Initials><AffiliationInfo><Affiliation>Centre for Microscopy, Characterisation and Analysis, The University of Western Australia, Perth, WA, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Thomas</LastName><ForeName>C</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>CARRTEL of Thonon-les-Bains, INRA, Thonon-les-Bains, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Martini</LastName><ForeName>R</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Department of Earth Sciences, University of Geneva, Geneva, Switzerland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ariztegui</LastName><ForeName>D</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>Department of Earth Sciences, University of Geneva, Geneva, Switzerland.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2016</Year><Month>09</Month><Day>30</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Geobiology</MedlineTA><NlmUniqueID>101185472</NlmUniqueID><ISSNLinking>1472-4669</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D002254">Carbonates</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D002254" MajorTopicYN="N">Carbonates</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000460" MajorTopicYN="N">Chlorophyta</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D003593" MajorTopicYN="N">Cytoplasm</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D060106" MajorTopicYN="N">Lakes</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D053858" MajorTopicYN="Y">Metabolic Networks and Pathways</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010839" MajorTopicYN="N">Phytoplankton</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013557" MajorTopicYN="N">Switzerland</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2016</Year><Month>04</Month><Day>27</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2016</Year><Month>08</Month><Day>25</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2016</Year><Month>10</Month><Day>4</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2017</Year><Month>9</Month><Day>7</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2016</Year><Month>10</Month><Day>4</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">27696636</ArticleId><ArticleId IdType="doi">10.1111/gbi.12213</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Allemagne</li><li>Australie</li><li>France</li><li>Suisse</li></country><region><li>Auvergne-Rhône-Alpes</li><li>Canton de Genève</li><li>Rhône-Alpes</li></region><settlement><li>Genève</li><li>Lyon</li><li>Thonon-les-Bains</li><li>Villeurbanne</li></settlement><orgName><li>Université de Genève</li></orgName></list><tree><country name="Suisse"><region name="Canton de Genève"><name sortKey="Martignier, A" sort="Martignier, A" uniqKey="Martignier A" first="A" last="Martignier">A. Martignier</name></region><name sortKey="Ariztegui, D" sort="Ariztegui, D" uniqKey="Ariztegui D" first="D" last="Ariztegui">D. Ariztegui</name><name sortKey="Barja, F" sort="Barja, F" uniqKey="Barja F" first="F" last="Barja">F. Barja</name><name sortKey="Filella, M" sort="Filella, M" uniqKey="Filella M" first="M" last="Filella">M. Filella</name><name sortKey="Jaquet, J M" sort="Jaquet, J M" uniqKey="Jaquet J" first="J-M" last="Jaquet">J-M Jaquet</name><name sortKey="Lavigne, S" sort="Lavigne, S" uniqKey="Lavigne S" first="S" last="Lavigne">S. Lavigne</name><name sortKey="Martini, R" sort="Martini, R" uniqKey="Martini R" first="R" last="Martini">R. Martini</name></country><country name="France"><region name="Auvergne-Rhône-Alpes"><name sortKey="Pacton, M" sort="Pacton, M" uniqKey="Pacton M" first="M" last="Pacton">M. Pacton</name></region><name sortKey="Thomas, C" sort="Thomas, C" uniqKey="Thomas C" first="C" last="Thomas">C. Thomas</name></country><country name="Allemagne"><noRegion><name sortKey="Pollok, K" sort="Pollok, K" uniqKey="Pollok K" first="K" last="Pollok">K. Pollok</name></noRegion><name sortKey="Langenhorst, F" sort="Langenhorst, F" uniqKey="Langenhorst F" first="F" last="Langenhorst">F. Langenhorst</name></country><country name="Australie"><noRegion><name sortKey="Guagliardo, P" sort="Guagliardo, P" uniqKey="Guagliardo P" first="P" last="Guagliardo">P. Guagliardo</name></noRegion><name sortKey="Kilburn, M R" sort="Kilburn, M R" uniqKey="Kilburn M" first="M R" last="Kilburn">M R Kilburn</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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